Enzymatic bypass and the structural basis of miscoding opposite the DNA adduct 1,N2-ethenodeoxyguanosine by human DNA translesion polymerase η
نویسندگان
چکیده
منابع مشابه
Regulation of translesion synthesis DNA polymerase by η monoubiquitination
DNA polymerase η is a Y-family polymerase involved in translesion synthesis (TLS). Its action is initiated by simultaneous interaction between the PIP box in polη and PCNA, and between the UBZ in polη and monoubiquitin attached to PCNA. While monoubiquitination of PCNA is required for its interaction with polη during TLS, we now show that monoubiquitination of polη inhibits this interaction, pr...
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Y-family DNA polymerases bypass Pt-GG, the cisplatin-DNA double-base lesion, contributing to the cisplatin resistance in tumour cells. To reveal the mechanism, we determined three structures of the Y-family DNA polymerase, Dpo4, in complex with Pt-GG DNA. The crystallographic snapshots show three stages of lesion bypass: the nucleotide insertions opposite the 3'G (first insertion) and 5'G (seco...
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Ribonucleotides (rNs) incorporated in the genome by DNA polymerases (Pols) are removed by RNase H2. Cytidine and guanosine preferentially accumulate over the other rNs. Here we show that human Pol η can incorporate cytidine monophosphate (rCMP) opposite guanine, 8-oxo-7,8-dihydroguanine, 8-methyl-2΄-deoxyguanosine and a cisplatin intrastrand guanine crosslink (cis-PtGG), while it cannot bypass ...
متن کاملComputational Evaluation of Nucleotide Insertion Opposite Expanded and Widened DNA by the Translesion Synthesis Polymerase Dpo4.
Expanded (x) and widened (y) deoxyribose nucleic acids (DNA) have an extra benzene ring incorporated either horizontally (xDNA) or vertically (yDNA) between a natural pyrimidine base and the deoxyribose, or between the 5- and 6-membered rings of a natural purine. Far-reaching applications for (x,y)DNA include nucleic acid probes and extending the natural genetic code. Since modified nucleobases...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2021
ISSN: 0021-9258
DOI: 10.1016/j.jbc.2021.100642